LND, POD time > 9 days, CEA > 7 ng/mL, CA 19–9 > 75 U/mL, operation time > 235 min, blood loss > 300 mL, intraoperative blood transfusion, tumor size > 6 cm, multi-disease, positive margin, vascular invasion, nerve invasion, T stage > T1, ALT > 50U/L, AST > 40U/L, TBIL > 21 μmol/L, and ALB < 35 g/L were risk factors for OS in univariate analysis. This evidence concerns the gene ALB and neoplasm.