In the present study, we adapted a droplet-based dPCR protocol previously described [13, 14] in the Naica Crystal Digital PCR systemTM (Stilla Technonologies, Villejuif, France) in order to investigate the hypermethylation of the NPY and WIF1 in CRC tissues or ctDNA. The gene discussed is NPY; the disease is colorectal carcinoma.