To understand mechanistically the hepatic steatosis development upon chronic IKK-mediated NF-κB activation, we quantified hepatic DNL and cholesterol synthesis by allowing incorporation of [1–13C]-acetate into fatty acids and cholesterol for five days, followed by mass isotopomer distribution analysis (MIDA) [34], in IKKβca;A20LKO mice and WT mice. This evidence concerns the gene NFKB1 and fatty liver disease.