Particularly, our results demonstrate that, despite the presence of a relatively hydrophobic BA pool, female Cyp2c70−/− mice are protected from WTD-induced obesity, insulin resistance and hepatic steatosis, whereas male Cyp2c70−/− mice show decreased hepatic fat accumulation but similar BW gain and insulin sensitivity than WT. This evidence concerns the gene INS and obesity due to melanocortin 4 receptor deficiency.