Then, exosome‐RNA‐sequencing (Exo‐RNA‐seq) was conducted and bioinformatic analysis results showed that dihydrofolate reductase (DHFR) was one of the most significant up‐regulated genes in exosomes derived from activated Lx‐2 cells when compared with quiescent Lx‐2 cells which suggested it could participate in liver fibrosis.23 This evidence concerns the gene DHFR and Hepatic fibrosis.