ITSN1 and neoplasm: The tumor volume was decreased in NLS-mutant ITSN1-S (shITSN1-S-3×flag-ITSN1-S-NLS-mutant/MDA-MB-231) and wild-type ITSN1-S (shITSN1-S-3×flag-ITSN1-S-WT/MDA-MB-231) mice groups compared with the control (shITSN1-S-3×flag-vector/MDA-MB-231 mice group), and wild-type ITSN1-S mice group exhibited the minimum xenografts (Fig. 4E).