For instance, given that Notch signaling is associated with MDSCs, TAMs and Tregs infiltration as well as immune checkpoint upregulation, flow cytometry analysis of a Tgfbr1/Pten-knockout head and neck squamous cell carcinoma mouse model indicates that blocking Notch1 with γ-secretase inhibitor could reduce the impact of the above factors on TME to reduce tumor burden [118]. This evidence concerns the gene PTEN and neoplasm.