MLKL and Alzheimer disease: Our results showing an increase in the expression of necroptosis pathway genes and proteins in the AD brain is consistent with previous studies on necroptosis activation [5, 30, 45], but build on this to show co-expression of RIPK1 and pRIPK3 with pMLKL in the same neurons, and coimmunoprecipitation of pRIPK3 with MLKL, indicating interaction and necrosome formation in these neurons.