PMEL and neoplasm: As a result, intratumoral injection of either autologous CD8+ T-cell melanoma antigen-activated by gp100-pulsed Flt3L-DCs or autologous CD4+ T cells activated by tumor lysate-pulsed Flt3L-DCs failed to mediate any measurable antitumor activity against pre-established B16F1 in B6 mice (Supplementary Fig. S2a-d).