CD8A and melanoma: To assess the specificity of these host CD8+ T cells against B16F1 melanoma, we isolated host CD8+ T cells from DLNs 24 h after AAA-CD4+ T-cell therapy and cultured them in a medium containing tumor cell lysates derived from a variety of B6 mouse tumor cell lines, including B16F1, Lewis lung carcinoma, C1498 leukemia, and EL4 lymphoma cells (Fig. 5c).