For this purpose, we selected a total of eight representative ALS-linked FUS mutants: two (P18S and A115N) within the N-terminal proximal QGSY-rich region; two (Δ173–174 and G206S) within the Gly-rich domain; two (R383C and M464I) within the split RGG domain; and two (R521C and P525L) within the C-terminal PY region containing nuclear localization signal (Fig. 4B). Here, FUS is linked to amyotrophic lateral sclerosis.