In addition, we observed an increased IL-12Rβ2, which account for mediating the immunosuppressive functions (42, 43), in splenic Tregs and aortic Tregs in hyperlipidemia status; this suggests that IL-35 signaling is induced in splenic ApoE–/– Tregs and atherosclerotic aortas. This evidence concerns the gene APOE and hyperlipidemia.