CTLA4 and autoimmune thyroid disease: As shown in Figure 7C, the top 10 gene classes enriched in ApoE–/– splenic Tregs included autoimmune thyroid disease, signaling by Hippo, insulin receptor recycling, P53 hypoxia pathway, TNF receptor–associated factor 6–mediated (TRAF6-mediated) IFN regulatory factor 7 (IRF-7) activation, CTLA-4 pathway, chemokine receptors bind chemokines, NKT pathway, retinol metabolism, and peptide ligand binding receptors.