In order to deeply explore the mechanism of AGPS and the relationship between AGPS and HNRNPK, we selected representative glioma U251 cells, hypothesized that AGPS can directly act on HNRNPK by Co-IP and mass spectrometry with the highest database score, and used confocal laser microscope to confirm that both are expressed in the nucleus, following study such as cell proliferation and content of tumor-related lipids also confirm this hypothesis. This evidence concerns the gene AGPS and glioma.