To investigate the conditions required for the induction of TRIM22 in A549 cells, we infected cells (WSN; MOI 0.01 PFU/cell) over an extended time course of infection to allow for multiple cycles of IAV replication that could stimulate the activation of IFN-mediated immune defences (Tapia et al., 2013; Killip et al., 2017; Russell et al., 2019). This evidence concerns the gene IFNA1 and infection.