Dual luciferase reporter assay revealed that miR-525-5p mimic transfection inhibited the activity of the luciferase reporter harboring the wild-type ARF6 3′UTR in chordoma cells, while co-transfection with lncRNA MDFIC-7 blocked the inhibitory effect of miR-525-5p mimic on luciferase activity (Figure 4C). Here, ARF6 is linked to chordoma.