To investigate whether ARF6 plays a similar role in chordoma cells and whether this function was modulated by the lncRNA MDFIC-7/miR-525-5p axis, we examined the status of ERK1/2 and the expressions of a series of glycolytic genes that encode key proteins with important roles in metabolizing glucose into lactate that are directly related to aerobic glycolysis, such as glucose transporter type 1 (GLUT1), hexokinase 2 (HK2), pyruvate dehydrogenase kinase 1 (PDK1) and lactate dehydrogenase A (LDHA) in U-CH1 and U-CH2 cells. The gene discussed is HK2; the disease is chordoma.