Our analysis using different methods has shown for the first time that autosomal genes NOX5, FRG1BP, and AL354714.2 and X-linked genes such as PUDP, ZFX, KDM6A, SYAP1, and DDX3X have been reported in different cancers to have differential DNA methylation and differential expression in males over females in GB. This evidence concerns the gene FRG1BP and cancer.