Since phosphorylated Smad3, N-cadherin, Snail and Vimentin were all increased in 3D-cultured ovarian cancer spheroids and E-cadherin was decreased in 3D-cultured ovarian cancer spheroids, we aimed to determine whether N-cadherin, Snail, Vimentin and E-cadherin are target molecules of the activated Smad3 pathway in 3D-cultured ovarian cancer spheroids. Here, CDH1 is linked to ovarian cancer.