Naked cuticle homolog 2 (Nkd2) small-interfering RNA (siRNA) and chemokine receptor 4 (CXCR4) expression plasmid were synthesized and transfected into curcumin-treated SW620 colorectal cancer cell lines, and the NKD2 and CXCR4 expression levels were determined. The gene discussed is NKD2; the disease is colorectal cancer.