HMGCS1 and colorectal cancer: We analyzed the correlation between stemness markers and cholesterol biosynthesis genes in TCGA colorectal cancer (COADREAD) cohort and revealed positive correlations between CSC markers (EphB2 and CD44) and cholesterol biosynthesis genes (HMGCR, HMGCS1, FDPS, and FDFT1) (Supplementary Fig. 3A).