AHSA1 and malignant colon neoplasm: To investigate whether AHA1 affects the proliferation, migration, and invasion of colon cancer cells, cell counting, wound healing, and transwell invasion assays were performed in SW480 and HCT116 cells with different expression levels of AHA1. To verify that AHA1 enhances cell migration and invasion abilities, overexpression of AHA1 by exogenously introduced AHA1-flag in SW480 cells, and knockdown of AHA1 by siAHA1 in HCT116 cells, were performed.