The endogenous expression levels of AHA1, Hsp90aa1, and Hsp90ab1 were examined in normal colon fibroblast cells (CCD18Co), early-stage colon cancer cells (HT-29), middle grade colon cancer cells (SW480, DLD-1), and highly metastatic colon cancer cells (Lovo, KM12SA, and HCT-116), using western blot and qRT-PCR analyses. The gene discussed is AHSA1; the disease is malignant colon neoplasm.