SLC2A1 and glioblastoma: To investigate the functions of DHHC9-mediated GLUT1 S-palmitoylation in GBM development, we intracranially injected athymic nude mice with luciferase-expressing U87 cells with or without knockout of endogenous DHHC9 or GLUT1 and reconstituted expression of their WT counterparts, rDHHC9 C169S or rGLUT1 C207S (Fig. 5a).