To investigate the functions of DHHC9-mediated GLUT1 S-palmitoylation in GBM development, we intracranially injected athymic nude mice with luciferase-expressing U87 cells with or without knockout of endogenous DHHC9 or GLUT1 and reconstituted expression of their WT counterparts, rDHHC9 C169S or rGLUT1 C207S (Fig. 5a). This evidence concerns the gene ZDHHC9 and glioblastoma.