BUB1B and cervical squamous intraepithelial neoplasia: Thirdly, chromosomal plate width and mitotic bipolar spindle length, another two key features of CIN18,19 were examined by immunofluorescent (IF) staining for α-Tubulin and DAPI, and it was shown that upregulation of BUB1B remarkably increased chromosomal plate width (p < 0.05) and decreased mitotic bipolar spindle length (p < 0.001) in MM cells (Fig. 3c, Supplementary Fig. 1b), revealing that amplification of BUB1B promoted CIN.