To elucidate the mechanism by which BUB1B affected CIN, Co-IP analysis followed by mass spectrometry (MS) was conducted to detect the BUB1B downstream targets, and Centrosomal Protein 170 (CEP170) was identified among the top of the detected proteins interacting with BUB1B (Fig. 3f). This evidence concerns the gene BUB1B and cervical squamous intraepithelial neoplasia.