To explore the potential function of PCK1 in pancreatic cancer cells, two lentiviral PCK1 shRNAs, with non-overlapping sequences (“sh-PCK1-Seq1/sh-PCK1-Seq2”), were individually transduced to PANC-1 cells and PATU-8988 cells. This evidence concerns the gene PCK1 and familial pancreatic carcinoma.