Among them, only the expression level of PD-L1 is insufficient regarding the prediction of the prognosis of immunotherapy in NSCLC (Reck et al. 2016; Taube et al. 2014), while TMB has been regarded as an appropriate predictive biomarker for immunotherapy efficacy in various cancers, such as NSCLC (Goodman et al. 2017; Gubin et al. 2015; McGranahan et al. 2016). This evidence concerns the gene CD274 and non-small cell lung carcinoma.