NLK and cancer: Due to the additional chromosomes that result from the nondiploid nature of the BV2 cancer cell line, incomplete Nlk gene-targeted BV2 cell clones were identified, and 1 clone was selected as Nlk-knockdown (Nlk-KD) cells for our analyses, which led to a consistent knockdown efficiency of Nlk (about 70% reduction) at the protein level (Figure 3, F and G) and a significant reduction at the mRNA level (Figure 3H).