In these AppNL-F mice, the Aβ sequence has been completely humanized and the early onset AD Swedish mutation (5’ located mutations encoding K670N and M671L = NL) and the Beyreuther/Iberian mutation (3’ flanking mutation encoding I716F = F) were also introduced, resulting in increased Aβ production, but physiological regulation of APP expression. This evidence concerns the gene APP and Alzheimer disease.