Further, to analyze the role of SRSF10 in PKM and BCLx pre-mRNA splicing, we examined the PKM and BCLx pre-mRNA-splicing pattern in SRSF10 depleted HNC cells and observed the splicing switch of PKM and BCLx gene from cancer-specific (PKM2 and BCLxL) isoform to normal isoform (PKM1 and BCLxs). This evidence concerns the gene PKM and cancer.