Indeed, several mutated genes in addition to BCR-ABL1 were identified by the DNA NGS myeloid panel in resistant KCL-22R clones, namely, BCOR, ATRX, RUNX1 and KRAS. All these genes were previously found to be mutated in CML, although their clinical importance for CML patients remains unclear (5, 16, 17). This evidence concerns the gene RUNX1 and chronic myelogenous leukemia, BCR-ABL1 positive.