By comparing the images of immunohistochemical staining in HNSCC tissue and normal tissues of the head and neck, we found that 11 DE-FRGs (ASNS, AURKA, FTH1, SLC2A3, SLC7A5, CISD2, PRDX6, ATG5, BAP1, MAP1LC3A, and SOCS1) showed differences in proteins expression (Figures 13A–K). Here, ASNS is linked to head and neck squamous cell carcinoma.