By comparing the images of immunohistochemical staining in HNSCC tissue and normal tissues of the head and neck, we found that 11 DE-FRGs (ASNS, AURKA, FTH1, SLC2A3, SLC7A5, CISD2, PRDX6, ATG5, BAP1, MAP1LC3A, and SOCS1) showed differences in proteins expression (Figures 13A–K). This evidence concerns the gene CISD2 and head and neck squamous cell carcinoma.