Mutation of TAOK1 of the Hippo pathway and KEAP1 of the NRF2 pathway in the progressive tumors of P3 as well as NOTCH1 (frameshift deletion of p.R1824fs) of Notch pathway, IRS1 and RASAL2 of RTK-RAS pathway, TP53 of p53/CPF pathway, and a multidrug-resistance gene ABCC11 in the progressive tumors of P8 were also detected, indicating their roles are involved in the evolution of tumor progression and possibly also are involved in drug resistance. This evidence concerns the gene IRS1 and neoplasm.