To test the impact of CLDN7 knockdown (KD) on hCMEC/D3 susceptibility to African ZIKV infection, hCMEC/D3 cells were transduced with lentiviral vectors expressing shRNA sequences targeting CLDN7 or, as controls, CLDN5 transcripts or non-targeting shRNAs (CTRL). This evidence concerns the gene CLDN7 and Zika virus infectious disease.