NOS3 and endothelial dysfunction: In the aortas of STZ-induced diabetic rats with endothelial dysfunction, the reduction in TRPV4 expression was accompanied by a reduction in both eNOS and caveolin-1 expression; insulin treatment reversed the endothelial dysfunction and was associated with the upregulation of TRPV4 expression, eNOS and caveolin-1 (Shamsaldeen et al., 2020).