TRPV4 and obesity due to melanocortin 4 receptor deficiency: In that study, they showed that both carbacol- and TRPV4 agonist-induced vasorelaxations were impaired in the third-order mesenteric arteries of male C57BL6/J mice with diet-induced (60% of total energy from fat for 14weeks) obesity (Ottolini et al., 2020); this was the result of increased peroxynitrite formation at MEPs, which in turn oxidized the regulatory protein AKAP150 to impair AKAP150-dependent cooperative TRPV4 activation without altering TRPV4 expression (Ottolini et al., 2020).