Calorie restriction (CR) is defined as a sustained decline in energy intake compared to pre-intervention requirements without causing malnutrition (typically 20–50% less than average), and it has been shown to mediate a life-extending effect in the lower organisms through a key molecule Sir2 which previously mentioned to be highly homologous to SIRT1 in mammals (Yang N.-C. Here, SIRT1 is linked to nutritional deficiency disease.