In the AOM/DSS model, colon epithelium-specific Znf545−/− mice (Znf545flox/floxCDX2P-CreERT2), Znf545−/+ mice (Znf545flox/+CDX2P-CreERT2), and wild type (WT) mice (CDX2P-CreERT2) were treated with tamoxifen, followed by carcinogen AOM and three cycles of DSS treatment to induce colon cancer (Fig. 1b). This evidence concerns the gene ZFP82 and infectious otitis media.