As expected, LPS-primed immortalized BMDMs expressing S803A, S803D or S803E mutants secreted equal amounts of IL-1β as compared to BMDMs expressing WT NLRP3 upon S. Typhimurium SL1344 infection or transfection with flagellin activating the NLRC4 inflammasome, as well as upon transfection with poly(dA:dT) activating the AIM2 inflammasome (Supplementary Fig. 3b–d). This evidence concerns the gene IL1B and infection.