On the opposite, LPS-primed U937 expressing NLRP3 S806A, D, or E mutants secreted IL-1β as WT U937 in response to infection by Salmonella enterica serovar Typhimurium (SL1344 strain) and transfection with poly(dA:dT), activating the NLRC4 and AIM2 inflammasomes respectively (Supplementary Fig. 2f, g). The gene discussed is AIM2; the disease is infection.