In established PANC-1 cells and other primary human pancreatic cancer cells (PanCa-2 and PanCa-3), NSD3 silencing by shNSD3-S5 (see Fig. 2) significantly increased caspase-3 activity (Fig. 3G) and TUNEL-positive nuclei ratio (Fig. 3H), indicating apoptosis activation. Here, NSD3 is linked to familial pancreatic carcinoma.