Western blot results showed that the PI3K/Akt/mTOR pathway was suppressed and that autophagy-related proteins, such as ATG5, Beclin1, and LC3II/LC3I, were activated when ECHS1 was knocked out in CRC cells, which could be resorted by Eligustat, an inhibitor of UGCG. This evidence concerns the gene AKT1 and colorectal carcinoma.