ECHS1 and colorectal carcinoma: Additionally, in vivo subcutaneous tumor formation in BALB/C nude mice supported a stimulatory effect of ECHS1 on CRC cell proliferation (0.1 ± 0.05 g vs. 0.4 ± 0.3 g, P < 0.01; Fig. 5D), while knocking out ECHS1 dramatically inhibited the growth of subcutaneous tumors (0.2 ± 0.05 g vs. 0.08 ± 0.05 g, P < 0.01; Fig. 5E).