The regulatory impact of YAP/TAZ on selected candidates associated with cell cycle control (CDK2, PLK1), DNA replication (MCM3, MCM7) and Hippo signaling (AJUBA, TEAD4) was confirmed in different CCA cell lines after combined YAP/TAZ silencing (Fig. 4c, Supplementary Figure S3B), illustrating that these target gene effects were not restricted to HUCCT-1 cells. This evidence concerns the gene PLK1 and cholangiocarcinoma.