Aberrant Wnt signaling which is triggered by UBE2T overexpression is a feature of advanced HCC characterized by the activation of other main oncogenic pathways such as AKT/mTOR, MAPK/ERK, and TGF‐β, which are affected differently in 2D and 3D conditions in many cancers including HCC [69, 70, 71]. This evidence concerns the gene TGFB1 and hepatocellular carcinoma.