HIF1A and neoplasm: Buchanan et al., using a 3D microfluidic breast tumor vascular model of MDA-MB-231 which showed that endothelial cells displayed enhanced permeability and tumor cells downregulated their expression of angiogenic genes such as MMP9, HIF-1, VEGF-A, ANG1 and ANG2 in response to high microvascular wall shear stress of 10dyn/cm2 revealing the effect of mechanical forces in tumor angiogenesis (Buchanan et al. 2014).