BRAF and neoplasm: In addition, quantification of BRAF VAF by dPCR assay eliminated issues commonly present in IHC, such as discrepancies in staining procedures among different platforms8,48 and discord in interpretation of the staining results.8,48,51,52 Of note, IHC is of limited value in biopsy specimens, since equivocal staining results frequently occur on direct smears or cell blocks with scant tumor cellularity or purity.49,52,53,54 In this study, BRAF variation was successfully identified in 26.7% of residual FNA biopsies.