Stroke triggered 2-5 fold upregulation of WNK proteins(isoforms 1, 2, 4), SPAK/OSR1 and NKCC1 proteins in the Ang II-infused hypertensive brainsafter stroke, which were located in cortical neurons and associated with nucleartranslocation of phospho-NF-kB protein and increased NF-κB recruitment on Wnk1,Wnk2, Wnk4, Spak and Nkcc1 gene promoters. The gene discussed is WNK4; the disease is stroke disorder.