LCN2 and infection: To furtherdetermine if astrocytes define a chronic inflammatory state, single cell RNA sequencingexperiments on bulk astro-cytes at various stages of infection were conducted to determineif similar populations are expressed during acute compared to chronic infection.Furthermore, to address the issue of detecting a pure RA population, we have created areporter mouse, Lcn2 CreERT2; Rosa26 lsl-tdTomato, thatwill allow for identification of RA subsets during infection for the first time, providing anovel tool for future use.