The majority of embryos injected with atp1a3a-SP-MO or atp1a3b-SP-MO exhibit a reduced level of atp1a3a (∼62%) or atp1a3b (∼66%) transcript and show severe hydrocephalus as a consequence of CSF build-up within the brain ventricle (Doğanlı et al., 2013). The gene discussed is TFF2; the disease is Hydrocephalus.