Since 5-FU is commonly used for CRC chemotherapy, we validated our observations by performing dose-response curves of 5-FU in HT29 and HCT116 cells with RNF6 overexpression, which showed that RNF6 significantly increased 72 h-IC50 of 5-FU in both cell lines (Fig. 6A). This evidence concerns the gene RNF6 and colorectal carcinoma.