Two human CRC organoids, PDO74 and PDO828, cultured in 3D Matrigel were transfected with empty vector or RNF6 plasmid and treated with pladienolide B. Overexpression of RNF6 also activates SF3B2 expression in primary CRC organoids (Fig. 5C). This evidence concerns the gene RNF6 and colorectal carcinoma.