TNFSF10 and Alzheimer disease: Concerning the activation status of microglia and related proinflammatory molecules, western blot analysis revealed that, while the expression of microglial marker Iba-1 and of TNF-α was substantially present in the retina of 3xTg-AD mice, anti-TNFSF10 treatment significantly blunted their expression (Fig. 6A, B).