To explain the pathological significance of the high expression of SPNS3 in AML, we not only undertook GO, KEGG pathway gene enrichment and GSEA to show the main biological involvement, but also closely analyzed the role of SPNS3 in sphingosine-1-phospahte (S1P) signaling pathway and similar to other phosphosphingolipids, regulates complex cellular function in the tumor microenvironment where it plays a role as a signaling molecule that is involved in cell-cell communication [29]. The gene discussed is SPNS3; the disease is acute myeloid leukemia.