Firstly, we found that SPNS3 expression varied in six variants of AML cell-lines; therefore, we chose the cell-line with the highest SPNS3 expression (MOLM-13) for further experiments (Figure 6(a)) where MOLM-13 was transfected with SPNS3-targeting shRNAs for 12 h. This evidence concerns the gene SPNS3 and acute myeloid leukemia.