AFP and cholangiocarcinoma: AFP protein expression has additionally been shown to be a prognostic marker, as such, we performed a multivariate analysis; when adding AFP gene expression to the model, Blast-Like and CCA-Like were still significant predictors of worse progression-free (HR = 1.9, p = 0.002 and HR = 1.6, p = 0.03, respectively) and Blast-Like was significant predictor of worse overall survival (HR = 3.5, p = 8e−10).