To evaluate the effects of p53 in primary sensitivity and acquired resistance to EGFR-TKIs in NSCLC cells with activating EGFR mutations, we generated p53 knockout cells, including PC-9 (EGFR-Del19, p53-R248Q), HCC827 (EGFR-Del19, p53-v218del), and H1975 (EGFR-L858R + T790M, p53-R273H), using CRISPR-Cas9 KO plasmids targeting the p53 gene. Here, EGFR is linked to non-small cell lung carcinoma.