In particular, the m.13565C > T/MT‐ND5 mutation in osteosarcoma 143B cybrids caused a partial mitochondrial depolarization and loss of TCA cycle enzyme regulation, thus affecting metabolism [86], while the m.13514A > G/MT‐ND5 mutation has been associated with increased AMPK‐mediated autophagy and faster mitochondrial turnover, as a compensatory mechanism relieving organelle dysfunction [87]. Here, MT-ND5 is linked to osteosarcoma.