The overall KRAS expression declined in HCC cells, while that of AKT and ERK1/2 (MAPK3) decreased only when the temperature was higher than 50 °C; in contrast, p-ERK1/2 expression was gradually upregulated when the temperature increased from 43 to 48 °C, and then, its overall expression decreased significantly (Fig. 8F). Here, MAPK3 is linked to hepatocellular carcinoma.