Thus, in this research, the mRNA and protein expression levels of LETM1 in ESCC cells were examined first and the results showed that the expression of LETM1 in ESCC cells was remarkably enhanced compared with that in esophageal epithelial cells, which was consistent with the results of tissue samples in the study by Yang [7]. Here, LETM1 is linked to esophageal squamous cell carcinoma.